Statement of problem
Adherence and colonization of Candida albicans on tissue conditioners is common and results in irritation of the denture-bearing
mucosa.
Purpose
The purpose of this study was to investigate the antifungal activity and properties
of a tissue conditioner by incorporating origanum oil.
Material and methods
Origanum oil at varying concentrations was incorporated into a poly(methyl methacrylate)
based tissue conditioner (Visco-gel), and its antifungal activity against Candida albicans was evaluated at 1 day and 1 week by using the agar punch well method. The adherence
of Candida albicans, surface roughness, tensile strength, and bond strength of the tissue conditioner
with an optimized origanum oil concentration were evaluated. The data were subjected
to 2-way ANOVA (α=.05).
Results
Sixty vol% origanum oil in tissue conditioner (Visco-gel) showed a mean inhibitory
zone of 21.00 ±1.58 mm at 1 day and 13.44 ±0.88 mm at 1 week. The control group showed
90 ±6.80 yeast cells/mm2 at 1 day and 165 ±7.63 yeast cells/mm2 at 1 week, whereas the group with origanum oil showed 16 ±1.15 yeast cells/mm2 at 1 day and 32 ±4.00 yeast cells/mm2 at 1 week. Surface roughness was less with the incorporation of origanum oil. Tensile
strength at 1 day was 0.91 ±0.52 N for the control group, whereas the group with origanum
oil showed 0.16 ±0.05 N. At 1 day, the bond strength of 3.97 ±0.75 MPa was observed
with control specimens, whereas tissue conditioner with origanum oil showed a bond
strength of 3.73 ±0.65 MPa.
Conclusions
Within the limitations of this in vitro study, origanum oil can be used as an additive
to tissue conditioner to reduce the adherence of Candida albicans without significantly affecting its bond strength to heat-polymerized acrylic resin
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© 2013 The Editorial Council of the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.
