Abstract
Statement of problem
Streptococcus mutans can adhere at restored tooth margins to cause recurrent caries. Limited information
about surface quality and bacterial adherence is available for lithium disilicate
ceramic materials.
Purpose
The purpose of this in vitro study was to investigate how bacterial adherence is influenced
by commercially available preparations of lithium disilicate ceramic materials.
Material and methods
Seventeen rectangular specimens (10×10×4 mm) were fabricated for each type of lithium
disilicate material: pressed (Press), milled (CAD), fluorapatite layered (ZirPress/Ceram),
and glazed (Ceram Glaze). The surface roughness of each specimen was assessed before
incubation with wild-type S mutans for 48 hours at 37°C with Brain Heart Infusion broth media under anaerobic conditions.
Adherent bacteria were sonicated, diluted, and plated in triplicate for quantification
using the plate count method to assay for colony forming units (CFUs) as an indication
of bacterial viability. Statistical analysis was performed with SPSS using an analysis
of variance (ANOVA) followed by the Tukey Honestly Significant Difference (HSD) test
(α=.05). The Pearson r was used to evaluate the correlation between surface roughness and adherence.
Results
The surface roughness of Ceram Glaze (1.32 ±0.19 μm) was significantly the highest,
followed by ZirPress/Ceram (0.71 ±0.09 μm), which was significantly rougher than the
Press (0.11 ±0.02 μm) and CAD (0.10 ±0.02 μm) groups, which were not significantly
different from each other. (F=513.898, P<.001). CFUs (cells/mL) of S mutans were also significantly the highest for Ceram Glaze (61.82 ±13.76), followed by ZirPress/Ceram
(28.53 ±2.40), which had significantly higher adherence than CAD (12.86 ±1.70) and
Press (6.62 ±2.74), which were not significantly different from each other. (F= 201.721,
P<.001). A strong positive association was found between bacterial count and surface
roughness (r=.95, P<.001).
Conclusions
The surface roughness of differently prepared lithium disilicate ceramic restorations
is closely related to the adherence of S mutans.
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Article info
Publication history
Published online: September 16, 2015
Footnotes
Supported by an American Academy of Fixed Prosthodontics Stanley D. Tylman Research Grant.
Identification
Copyright
© 2015 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.
