Abstract
Statement of problem
Although the physical and mechanical properties of hydroxyapatite-filled dental restorative
composite resins have been examined, the biocompatibility of these materials has not
been studied in detail.
Purpose
The purpose of this in vitro study was to analyze the toxicity of acrylate-based restorative
composite resins filled with hydroxyapatite and a silica/hydroxyapatite combination.
Material and methods
Five different restorative materials based on bisphenol A-glycidyl methacrylate (bis-GMA)
and tri-ethylene glycol dimethacrylate (TEGDMA) were developed: unfilled (H0), hydroxyapatite-filled
(H30, H50), and silica/hydroxyapatite-filled (SH30, SH50) composite resins. These
were tested for in vitro cytotoxicity by using human bone marrow mesenchymal stromal
cells. Surface morphology, elemental composition, and functional groups were determined
by scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDX),
and Fourier-transformed infrared spectroscopy (FTIR). The spectra normalization, baseline
corrections, and peak integration were carried out by OPUS v4.0 software.
Results
Both in vitro cytotoxicity results and SEM analysis indicated that the composite resins developed
were nontoxic and supported cell adherence. Elemental analysis with EDX revealed the
presence of carbon, oxygen, calcium, silicon, and gold, while the presence of methacrylate,
hydroxyl, and methylene functional groups was confirmed through FTIR analysis.
Conclusions
The characterization and compatibility studies showed that these hydroxyapatite-filled
and silica/hydroxyapatite-filled bis-GMA/TEGDMA-based restorative composite resins
are nontoxic to human bone marrow mesenchymal stromal cells and show a favorable biologic
response, making them potential biomaterials.
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Article info
Publication history
Published online: February 10, 2016
Footnotes
Supported by grant BT/PR11096/MED/32/40/2008 from the Department of Biotechnology, Government of India, New Delhi; and grants from the High Impact Research, Ministry of Higher Education (HIR-MOHE, E000003-20001), University of Malaya, for funding in support of the cytotoxicity tests.
Identification
Copyright
Copyright © 2016 by the Editorial Council for The Journal of Prosthetic Dentistry.
