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Journal of Prosthetic Dentistry

Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces

Published:February 07, 2020DOI:https://doi.org/10.1016/j.prosdent.2019.11.024

      Abstract

      Statement of problem

      Changes in physicochemical properties because of implant material aging and natural deterioration in the oral environment can facilitate microbial colonization and disturb the soft-tissue seal between the implant surfaces.

      Purpose

      The purpose of this in vitro study was to investigate the effect of aging time on the physicochemical profile of titanium (Ti) and zirconia (ZrO2) implant materials. Further microbiology and cell analyses were used to provide insights into the physicochemical implications of biological behavior.

      Material and methods

      Disk-shaped specimens of Ti and ZrO2 were submitted to roughness, morphology, and surface free energy (SFE) analyses before nonaging (NA) and after the aging process (A). To simulate natural aging, disks were subjected to low-temperature degradation (LTD) by using an autoclave at 134 ºC and 0.2 MPa pressure for 20 hours. The biological activities of the Ti and ZrO2 surfaces were determined by analyzing Candida albicans (C. albicans) biofilms and human gingival fibroblast (HGF) cell proliferation. For the microbiology assays, a variance analysis method (ANOVA) was used with the Tukey post hoc test. For the evaluation of cellular proliferation, the Kruskal-Wallis test followed by Dunn multiple comparisons were used.

      Results

      Ti nonaging (TNA) and ZrO2 nonaging (ZNA) disks displayed hydrophilic and lipophilic properties, and this effect was sustained after the aging process. Low-temperature degradation resulted in a modest change in intermolecular interaction, with 1.06-fold for TA and 1.10-fold for ZA. No difference in biofilm formation was observed between NA and A disks of the same material. After 48 hours, the viability of the attached HGF cells was very similar to that in the NA and A groups, regardless of the tested material.

      Conclusion

      The changes in the physicochemical properties of Ti and ZrO2 induced by the aging process do not interfere with C. albicans biofilm formation and HGF cell attachment, even after long-term exposure.
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      References

        • Paster B.J.
        • Boches S.K.
        • Galvin J.L.
        • Ericson R.E.
        • Lau C.N.
        • Levanos V.A.
        • et al.
        Bacterial diversity in human subgingival plaque.
        J Bacteriol. 2001; 183: 3770-3783
        • Choi S.H.
        • Jeong W.S.
        • Cha J.Y.
        • Lee J.H.
        • Lee K.J.
        • Yu H.S.
        • et al.
        Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment.
        Sci Rep. 2017; 7: 3833
        • Correa D.R.N.
        • Kuroda P.A.B.
        • Lourenco M.L.
        • Buzalaf M.A.R.
        • Mendoza M.E.
        • Archanjo B.S.
        • et al.
        Microstructure and selected mechanical properties of aged Ti-15Zr-based alloys for biomedical applications.
        Mater Sci Eng C Mater Biol Appl. 2018; 91: 762-771
        • Papanagiotou H.P.
        • Morgano S.M.
        • Giordano R.A.
        • Pober R.
        In vitro evaluation of low-temperature aging effects and finishing procedures on the flexural strength and structural stability of Y-TZP dental ceramics.
        J Prosthet Dent. 2006; 96: 154-164
        • Pereira G.K.R.
        • Muller C.
        • Wandscher V.F.
        • Rippe M.P.
        • Kleverlaan C.J.
        • Valandro L.F.
        Comparison of different low-temperature aging protocols: its effects on the mechanical behavior of Y-TZP ceramics.
        J Mech Behav Biomed Mater. 2016; 60: 324-330
        • Pereira G.K.R.
        • Silvestri T.
        • Amaral M.
        • Rippe M.P.
        • Kleverlaan C.J.
        • Valandro L.F.
        Fatigue limit of polycrystalline zirconium oxide ceramics: Effect of grinding and low-temperature aging.
        J Mech Behav Biomed Mater. 2016; 61: 45-54
        • de Avila E.D.
        • Avila-Campos M.J.
        • Vergani C.E.
        • Spolidorio D.M.
        • Mollo Fde Jr., A.
        Structural and quantitative analysis of a mature anaerobic biofilm on different implant abutment surfaces.
        J Prosthet Dent. 2016; 115: 428-436
        • de Avila E.D.
        • de Molon R.S.
        • Lima B.P.
        • Lux R.
        • Shi W.
        • Junior M.J.
        • et al.
        Impact of physical chemical characteristics of abutment implant surfaces on bacteria adhesion.
        J Oral Implantol. 2016; 42: 153-158
        • de Avila E.D.
        • de Molon R.S.
        • Palomari Spolidorio D.M.
        • de Assis Mollo Jr., F.
        Implications of surface and bulk properties of abutment implants and their degradation in the health of periodontal tissue.
        Materials (Basel). 2013; 6: 5951-5966
        • de Avila E.D.
        • de Molon R.S.
        • Vergani C.E.
        • Mollo Jr., F.A.
        • Salih V.
        The relationship between biofilm and physical-chemical properties of implant abutment materials for successful dental implants.
        Materials (Basel). 2014; 7: 3651-3662
        • de Avila E.D.
        • Vergani C.E.
        • Mollo Junior F.A.
        • Junior M.J.
        • Shi W.
        • Lux R.
        Effect of titanium and zirconia dental implant abutments on a cultivable polymicrobial saliva community.
        J Prosthet Dent. 2017; 118: 481-487
        • Hayashi R.
        • Ueno T.
        • Migita S.
        • Tsutsumi Y.
        • Doi H.
        • Ogawa T.
        • et al.
        Hydrocarbon deposition attenuates osteoblast activity on titanium.
        J Dent Res. 2014; 93: 698-703
        • Zhang Y.
        • Addison O.
        • Yu F.
        • Troconis B.C.R.
        • Scully J.R.
        • Davenport A.J.
        Time-dependent enhanced corrosion of Ti6Al4V in the presence of H2O2 and albumin.
        Sci Rep. 2018; 8: 3185
        • Wang J.L.
        • Liu R.L.
        • Majumdar T.
        • Mantri S.A.
        • Ravi V.A.
        • Banerjee R.
        • et al.
        A closer look at the in vitro electrochemical characterisation of titanium alloys for biomedical applications using in-situ methods.
        Acta Biomater. 2017; 54: 469-478
        • Att W.
        • Hori N.
        • Takeuchi M.
        • Ouyang J.
        • Yang Y.
        • Anpo M.
        • et al.
        Time-dependent degradation of titanium osteoconductivity: an implication of biological aging of implant materials.
        Biomaterials. 2009; 30: 5352-5363
        • Denry I.
        • Kelly J.R.
        Emerging ceramic-based materials for dentistry.
        J Dent Res. 2014; 93: 1235-1242
        • Chevalier J.
        • Loh J.
        • Gremillard L.
        • Meille S.
        • Adolfson E.
        Low-temperature degradation in zirconia with a porous surface.
        Acta Biomater. 2011; 7: 2986-2993
        • Hallmann L.
        • Ulmer P.
        • Wille S.
        • Kern M.
        Effect of differences in coefficient of thermal expansion of veneer and Y-TZP ceramics on interface phase transformation.
        J Prosthet Dent. 2014; 112: 591-599
        • Scherrer S.S.
        • Cattani-Lorente M.
        • Vittecoq E.
        • de Mestral F.
        • Griggs J.A.
        • Wiskott H.W.
        Fatigue behavior in water of Y-TZP zirconia ceramics after abrasion with 30 mum silica-coated alumina particles.
        Dent Mater. 2011; 27: 28-42
        • Amaral M.
        • Valandro L.F.
        • Bottino M.A.
        • Souza R.O.
        Low-temperature degradation of a Y-TZP ceramic after surface treatments.
        J Biomed Mater Res B Appl Biomater. 2013; 101: 1387-1392
        • Cattani-Lorente M.
        • Durual S.
        • Amez-Droz M.
        • Wiskott H.W.
        • Scherrer S.S.
        Hydrothermal degradation of a 3Y-TZP translucent dental ceramic: A comparison of numerical predictions with experimental data after 2 years of aging.
        Dent Mater. 2016; 32: 394-402
        • Lughi V.
        • Sergo V.
        Low temperature degradation -aging- of zirconia: A critical review of the relevant aspects in dentistry.
        Dent Mater. 2010; 26: 807-820
        • Ritter J.E.
        Predicting lifetimes of materials and material structures.
        Dent Mater. 1995; 11: 142-146
        • Zhang Y.
        • Lawn B.
        Long-term strength of ceramics for biomedical applications.
        J Biomed Mater Res B Appl Biomater. 2004; 69: 166-172
        • Pereira G.
        • Amaral M.
        • Cesar P.F.
        • Bottino M.C.
        • Kleverlaan C.J.
        • Valandro L.F.
        Effect of low-temperature aging on the mechanical behavior of ground Y-TZP.
        J Mech Behav Biomed Mater. 2015; 45: 183-192
        • Miyauchi T.
        • Yamada M.
        • Yamamoto A.
        • Iwasa F.
        • Suzawa T.
        • Kamijo R.
        • et al.
        The enhanced characteristics of osteoblast adhesion to photofunctionalized nanoscale TiO2 layers on biomaterials surfaces.
        Biomaterials. 2010; 31: 3827-3839
        • Esfahanizadeh N.
        • Motalebi S.
        • Daneshparvar N.
        • Akhoundi N.
        • Bonakdar S.
        Morphology, proliferation, and gene expression of gingival fibroblasts on laser-lok, titanium, and zirconia surfaces.
        Lasers Med Sci. 2016; 31: 863-873
        • Nevins M.
        • Kim D.M.
        • Jun S.H.
        • Guze K.
        • Schupbach P.
        • Nevins M.L.
        Histologic evidence of a connective tissue attachment to laser microgrooved abutments: a canine study.
        Int J Periodontics Restorative Dent. 2010; 30: 245-255
        • Rutkunas V.
        • Bukelskiene V.
        • Sabaliauskas V.
        • Balciunas E.
        • Malinauskas M.
        • Baltriukiene D.
        Assessment of human gingival fibroblast interaction with dental implant abutment materials.
        J Mater Sci Mater Med. 2015; 26: 169
        • Beaussart A.
        • Alsteens D.
        • El-Kirat-Chatel S.
        • Lipke P.N.
        • Kucharíková S.
        • Van Dijck P.
        • et al.
        Single-molecule imaging and functional analysis of Als adhesins and mannans during Candida albicans morphogenesis.
        ACS Nano. 2012; 6: 10950-10964
        • Glee P.M.
        • Sundstrom P.
        • Hazen K.C.
        Expression of surface hydrophobic proteins by Candida albicans in vivo.
        Infect Immun. 1995; 63: 1373-1379
        • Kamo M.
        • Kyomoto M.
        • Miyaji F.
        Time course of surface characteristics of alkali- and heat-treated titanium dental implants during vacuum storage.
        J Biomed Mater Res B Appl Biomater. 2017; 105: 1453-1460
        • Spriano S.
        • Sarath Chandra V.
        • Cochis A.
        • Uberti F.
        • Rimondini L.
        • Bertone E.
        • et al.
        How do wettability, zeta potential and hydroxylation degree affect the biological response of biomaterials?.
        Mater Sci Eng C Mater Biol Appl. 2017; 74: 542-555
        • Song F.
        • Koo H.
        • Ren D.
        Effects of material properties on bacterial adhesion and biofilm formation.
        J Dent Res. 2015; 94: 1027-1034
        • Bollen C.M.
        • Papaioanno W.
        • Van Eldere J.
        • Schepers E.
        • Quirynen M.
        • van Steenberghe D.
        The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis.
        Clin Oral Implants Res. 1996; 7: 201-211
        • Rigolin M.S.M.
        • de Avila E.D.
        • Basso F.G.
        • Hebling J.
        • de S.C.C.A.
        • Mollo Junior F.A.
        Effect of different implant abutment surfaces on OBA-09 epithelial cell adhesion.
        Microsc Res Tech. 2017; 80: 1304-1309
        • de Avila E.D.
        • Lima B.P.
        • Sekiya T.
        • Torii Y.
        • Ogawa T.
        • Shi W.
        • et al.
        Effect of UV-photofunctionalization on oral bacterial attachment and biofilm formation to titanium implant material.
        Biomaterials. 2015; 67: 84-92
        • Hori N.
        • Iwasa F.
        • Tsukimura N.
        • Sugita Y.
        • Ueno T.
        • Kojima N.
        • et al.
        Effects of UV photofunctionalization on the nanotopography enhanced initial bioactivity of titanium.
        Acta Biomater. 2011; 7: 3679-3691
        • Wille S.
        • Zumstrull P.
        • Kaidas V.
        • Jessen L.K.
        • Kern M.
        Low temperature degradation of single layers of multilayered zirconia in comparison to conventional unshaded zirconia: phase transformation and flexural strength.
        J Mech Behav Biomed Mater. 2018; 77: 171-175
        • Pereira G.K.R.
        • Venturini A.B.
        • Silvestri T.
        • Dapieve K.S.
        • Montagner A.F.
        • Soares F.Z.M.
        • et al.
        Low-temperature degradation of Y-TZP ceramics: A systematic review and meta-analysis.
        J Mech Behav Biomed Mater. 2015; 55: 151-163
        • Zisman W.A.
        Relation to the equilibrium contact angle to liquid and solid constitution.
        Adv Chem. 1964; : 1-51
        • Owens D.K.
        • Wendt R.C.
        Estimation of the surface free energy of polymers.
        J Appl Polym Sci. 1969; 13: 1741-1747
        • Pellissari C.V.
        • Pavarina A.C.
        • Bagnato V.S.
        • Mima E.G.
        • Vergani C.E.
        • Jorge J.H.
        Cytotoxicity of antimicrobial photodynamic inactivation on epithelial cells when co-cultured with Candida albicans.
        Photochem Photobiol Sci. 2016; 15: 682-690