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Journal of Prosthetic Dentistry
Research and Education| Volume 127, ISSUE 1, P134-140, January 2022

Dental student, dentist, dental assistant, and layperson perception of pink gingival porcelain color

Published:November 21, 2020DOI:https://doi.org/10.1016/j.prosdent.2020.08.024

      Abstract

      Statement of problem

      How pink gingival porcelain color is perceived by participants classified according to profession and sex is unclear.

      Purpose

      The purpose of this in vitro study was to evaluate the degree of correlation between the ordering of color tabs by participants classified according to profession, years of dental work experience, and sex and the ideal chromatic order based on the color coordinate L∗ (lightness).

      Material and methods

      Twenty-one colored specimens made of Heraceram pink gingival porcelain were used. A group of 352 participants (118 men and 234 women) comprised of dental students (n=126), dentists (n=54), dental assistants (n=56), and laypersons (n=116) were enrolled. They were asked to order the specimens starting from the lightest to the darkest. The Kendall coefficients of concordance W and correlation T were used to determine whether the individuals in each experimental group rating the specimens were consistent and accurate.

      Results

      The degree of agreement between the ideal ordering and that carried out by the participants within the different professional categories was greater than 0.8 in all assessments. No statistically significant differences were found among the groups in relation to professional category, work experience, or sex (P>.05). The Kendall coefficients were higher in the ranking of the darker specimens for all groups of participants.

      Conclusions

      No statistically significant differences were found among dentists, dental assistants, dental students, and laypersons in the arrangement of pink porcelain specimens according to their lightness. In addition, in all groups, the darker gingival specimens were ordered significantly better according to their lightness than the lighter specimens.
      Clinical Implications
      Making a correct color selection is more likely for darker gingivae, regardless of sex, professional category, or years of dental work experience.
      Chromatic perception is a subjective and individual process.
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      but these studies have lacked a consistent finding.
      To achieve a high level of esthetic quality, the gingival color is important,
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      especially as approximately 80% of the population shows gingival tissue when smiling.
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      although gingival color is often included in removable dentures and implant-supported prostheses, particularly in patients with large defects, or anterior bone resorption.
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      The restoration color is most often selected subjectively,
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      • Buzello A.M.
      Interexaminer reliability in clinical measurement of L∗C∗h∗ values of anterior teeth using a spectrophotometer.
      but a standard gingival color guide is lacking. The lack of a reference guide impedes gingival color selection and communication with the dental laboratory technician. Therefore, studies that analyze the perception of gingival specimens among different population groups are needed because color selection is a critical factor for the success of esthetic restorations.
      • Montero J.
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      • Barrios R.
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      • López-Valverde A.
      The role of personality traits in self-rated oral health and preferences for different types of flawed smiles.
      Spectrophotometers can be used together with the color coordinates of the CIELab space to measure gingival color in a more objective manner.
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      Clinical study on natural gingival color.
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      In the CIELab space, lightness (L∗ coordinate) quantifies the amount of black and white, where pure black is L∗=0 and pure white is L∗=100; a∗ coordinate represents the amount of red-green, where positive a∗ indicates the amount of red and negative a∗ indicates the amount of green; b∗ coordinate is the yellow-blue measurement axis, where positive b∗ indicates the amount of yellow and negative b∗ indicates the amount of blue.
      CIE (Commission Internationale de l’Éclairage)
      Annuaire, roster, register, annexeau bulletin CIE.
      Lightness is the most highly recognizable color in the measurement of visual color.
      • Gómez-Polo C.
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      ,
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      Craig's restorative dental materials.
      However, the CIELab color space is not a uniform space, and the perception of teeth is not necessarily identical to the perception of gingival space color.
      The objective of the present study was to determine whether the professional category (students, dentists, dental assistants, laypersons), the sex, and the years of dental professional experience (0 years, 1-10 years, more than 10 years) influence the ability to correctly sort pink ceramic specimens according to their degree of lightness. The null hypotheses were that none of the 3 variables would significantly influence the accuracy level of participants in the arrangement of pink ceramic specimens and that the participants would be as accurate when ordering lighter specimens as when doing so with darker ones.

      Material and methods

      A total of 21 different colored specimens made of pink gingiva Heraceram porcelain (Heraceram; Kulzer GmbH) were used in this study. The specimens were made from a 10.6×61.6×33.2-mm silicone template (New Architect wax-up assistant anterior Form B Large; SmileLine Europe GmbH), obtained from Kulzer GmbH. The 21 specimens comprised the 6 basic gingival colors of the Heraceram gingiva system and an additional 15 specimens by mixing the basic colors, as shown in Table 1. Porcelain dispensers (dosifiers; Renfert) were used to ensure the accuracy of the mixtures (Fig. 1).
      Table 1CIELab color coordinates of 21 pink porcelain specimens
      Pink Gingival Specimens (CIELab Color Coordinates)
      Porcelain Specimen MixturesL∗a∗b∗
      100% G261.321.213.0
      100% G436.833.512.4
      100% G556.725.78.0
      100% G660.220.913.6
      100% G758.124.08.9
      100% G848.929.913.8
      75% G2 + 25% G456.822.417.4
      50% G2 + 50% G445.232.812.5
      25% G2 + 75% G457.724.710.9
      75% G4 + 25% G557.022.617.7
      50% G4 + 50% G555.928.46.2
      25% G4 + 75% G541.533.016.4
      75% G5 + 25% G644.732.613.7
      50% G5 + 50% G663.916.421.6
      25% G5 + 75% G637.732.712.9
      75% G6 + 25% G745.033.015.1
      50% G6 + 50% G761.718.618.0
      25% G6 + 75% G745.032.716.6
      75% G7 + 25% G843.135.219.4
      50% G7 + 50% G839.334.115.8
      25% G7 + 75% G860.121.211.1
      Figure thumbnail gr1
      Figure 1Color shade tab specimens made of pink gingiva Heraceram porcelain.
      A spectrometer (SpectroShade Micro; MHT OpticResearch AG) with a configuration of 45 degrees illumination and 0 degrees observation was used for the color measurements and was tested for reliability and precision. The 21 pink porcelain specimens were measured 3 times with the spectrophotometer in a neutral gray environment after calibration and following the manufacturer's instructions. The mean color coordinates were recorded.
      Dental students with previous training in the theory and practice in gingival shade matching were in charge of collecting the data. Each of the students sampled a minimum of 15 participants (without alterations in color perception and without cognitive impairment) in one or more of the professional groups of study (dentists, dental assistants, dental students, or laypersons). Each participant was shown 21 pink specimens on a gray cardboard backing and asked to sort from lighter to darker. The participants were allowed to carry out the task with no time restriction and under standardized conditions in the treatment room of the Department of Prosthodontics, School of Odontology. The ambient lighting was calibrated by using a Philips TLD 95 fluorescent daylight that provided an illumination of 5000 to 5500 K.
      The study included 352 participants (118 men and 234 women). The participants included dental students (n=126), dentists (n=54), dental assistants (n=56), and laypersons (n=116). In addition, the individuals were further divided into 3 subgroups based on dental work experience: those with no experience (n=116), those with 1 to 10 years of experience (n=186), and those with more than 10 years of experience (n=50). The study was evaluated by the Bioethics Committee of the University, and a consent form was provided and signed by all the participants.
      The Kendall coefficient of concordance W was used to evaluate the extent of agreement among the rankings of the 21 tabs by each experimental group of raters, based on lightness. Kendall W varied from 0 to 1, with 0 representing total lack of agreement and 1 representing total agreement. Thus, the association becomes stronger as the value of Kendall W increases.
      • Gwet K.L.
      Handbook of inter-rater reliability: the definitive guide to measuring the extent of agreement among raters.
      A Kendall coefficient of 0.9 or greater is considered as a high level of agreement; a high Kendall coefficient W means that the raters were applying essentially the same standard when assessing the shade tabs. Moreover, the Kendall correlation coefficient T was used to determine whether the raters of each experimental group were accurate. This coefficient, ranging from -1 to 1, was estimated by calculating the average of the Kendall tau coefficients of the rankings given by each rater and the known standard (the ranking of the 21 disks obtained using the L∗ coordinate). A positive value indicated a positive association and a negative value indicated a negative association, and the higher the magnitude, the stronger the association.
      • Agresti A.
      Analysis of ordinal categorical data.
      To compare the Kendall correlation coefficients among the groups of each category of the variables sex, professional category, and work experience, Mann-Whitney, Wilcoxon, and Kruskal-Wallis tests were applied (α=.05).

      Results

      The 21 specimens of pink porcelain used in this study showed maximum and minimum color differences according to the Euclidean formula of ΔE∗=33.34 units and ΔE∗=0.41 units. In terms of lightness, the maximum and minimum differences between these specimens were ΔL∗=27.04 and ΔL∗=0.03.
      All the W values were greater than 0.9, which indicated high levels of interrater agreement with respect to the assessment of the lightness of the 21 shade tabs (Table 2). Men were slightly less consistent than women, and the dentists were slightly less consistent than the dental students, dental assistants, and laypersons. The W values of the 3 subgroups based on work experience in dentistry were also similar. Table 3 lists the values calculated by using the Kendall correlation coefficient (T) on the results of the rankings obtained by the experimental groups and the rankings obtained objectively using the L∗ coordinates. Table 3 also includes the P values obtained when comparing the correlation coefficients among the groups of each category.
      Table 2Kendall W concordance coefficients to assess degree of coincidence of arrangements of all specimens in each group of participants
      VariableExperimental GroupnW
      Sex
      Men1180.920
      Women2340.937
      Professional category
      Students1260.935
      Dentists540.918
      Dental assistants560.937
      Laypersons1160.934
      Dental work experience
      0 years1160.934
      From 1 to 10 years1860.932
      More of 10 years500.927
      All participants3520.931
      Table 3Kendall T correlation coefficients to assess, in each group of participants, degree of coincidence of arrangements of all specimens with ideal arrangement
      VariableExperimental GroupnTP
      Sex.047
      Men1180.839
      Women2340.851
      Professional category.871
      Students1260.849
      Dentists540.839
      Dental assistants560.847
      Laypersons1160.847
      Dental work experience.763
      0 years1160.847
      From 1 to 10 years1860.848
      More of 10 years500.841
      All participants3520.847
      P values of intergroup comparisons of correlation coefficients T.
      The T values were all above 0.80, which indicated that the raters were moderately accurate. The T value calculated for the men was smaller than the value calculated for the women, and the difference was significant (P =.047). The T values calculated for the dental students, dentists, dental assistants, and laypersons lacked statistical significance (P>.05). Finally, no significant differences were observed for the T values of the 3 subgroups of participants divided according to dental work experience (Table 3) because those values were almost identical.
      The same analysis was repeated by using the 10 lighter specimens which had maximum and minimum color differences, according to the Euclidean formula, of ΔE∗=17.98 units and ΔE∗=0.41 units; the lightness (L∗ coordinate) of these 10 specimens had maximum and minimum differences of ΔL∗=7.14 units and ΔL∗=0.07 units, respectively. The T values obtained from the partial rankings of these 10 tabs (L∗, from 56.73 to 63.87) are shown in Table 4.
      Table 4Kendall T correlation coefficients to assess, in each group of participants, degree of coincidence of lighter specimens’ arrangements with ideal arrangement.
      VariableExperimental GroupnTP
      Sex.009
      Men1180.585
      Women2340.638
      Professional category.278
      Students1260.642
      Dentists540.573
      Dental assistants560.629
      Laypersons1160.615
      Dental work experience.431
      0 years1160.615
      From 1 to 10 years1860.627
      More of 10 years500.607
      All participants3520.620
      Pvalues of intergroup comparisons of correlation coefficients T.
      All the T values in Table 4 were significantly smaller than those in Table 3. In addition, the values calculated for male and female participants were significantly different (P=.009), with smaller values recorded for men. The values for the 4 professional categories were statistically similar, as were the groups in terms of dental work experience (I>0.05) (Table 4).
      The 10 darker specimens had maximum and minimum color differences, according to the Euclidean formula, of ΔE∗=12.70 units and ΔE∗=1.30 units, respectively; the lightness (L∗ coordinate) of these specimens had maximum and minimum differences of ΔL∗=12.10 and ΔL∗=∗0.03, respectively. The values T obtained from the rankings of these shade tabs (L∗, from 36.83 to 48.93) are shown in Table 5.
      Table 5Kendall T correlation coefficients to assess, in each group of participants, degree of coincidence of darker specimens’ arrangements with ideal arrangement
      VariableExperimental GroupnTP
      Sex.813
      Men1180.762
      Women2340.754
      Professional category.763
      Students1260.754
      Dentists540.774
      Dental assistants560.754
      Laypersons1160.755
      Dental work experience.939
      0 years1160.755
      From 1 to 10 years1860.759
      More of 10 years500.755
      All participants3520.757
      P values of intergroup comparisons of correlation coefficients T.
      The T values in Table 5 were found to be slightly smaller than those of Table 3 and greater than those of Table 4. This indicated that the raters were more accurate in ranking the darker tabs than the lighter ones. In Table 5, all the values were found to be similar and showed no statistically significant differences (P>.05).
      Figure 2 shows the comparison of the Kendall correlation coefficient (T) values calculated from the rankings of the 10 lighter shade tabs to the 10 darker shade tabs by the different experimental groups associated with the 3 variables: sex, work experience, and professional category. All the T values were greater for the darker tabs than those for the lighter ones in every experimental group. All the differences were statistically significant (P<.05).
      Figure thumbnail gr2a
      Figure 2Comparison of the T coefficients obtained when the lighter and darker specimens were ranked. A, By men and women. B, By participants with different levels of dental work experience. C, By participants of different professional categories.
      Figure thumbnail gr2b
      Figure 2Comparison of the T coefficients obtained when the lighter and darker specimens were ranked. A, By men and women. B, By participants with different levels of dental work experience. C, By participants of different professional categories.
      Figure thumbnail gr2c
      Figure 2Comparison of the T coefficients obtained when the lighter and darker specimens were ranked. A, By men and women. B, By participants with different levels of dental work experience. C, By participants of different professional categories.

      Discussion

      This study assessed the ability to perceive different degrees of lightness in custom-made pink ceramics shade tabs by a group of participants with different professional profiles and clinical work experience. Limitations of this study included that both the participants and specimens were convenience samples, indicating that the results obtained cannot be generalized to the global population. The 21 specimens of pink porcelain used offered a wide chromatic range, although these shade tabs may not fully represent the true chromatic range of the natural gingival tissue, whose chromatic space has not yet been documented.
      • Gómez-Polo C.
      • Montero J.
      • Gómez-Polo M.
      • Martín Casado A.M.
      Clinical study on natural gingival color.
      ,
      • Gómez Polo C.
      • Montero J.
      • Martín Casado A.M.
      Comparison of two color-difference formulas using the Bland-Altman approach based on gingiva color space.
      In addition, no reference guide for gingival color is currently in use within the scientific community,
      • Gómez-Polo C.
      • Montero J.
      • Gómez-Polo M.
      • Martín Casado A.M.
      Clinical study on natural gingival color.
      ,
      • Ghinea R.
      • Herrera L.J.
      • Pérez M.M.
      • Ionescu A.M.
      • Paravina R.D.
      Gingival shade guides: colorimetric and spectral modeling.
      making it difficult for dental laboratory technicians to imitate the natural gingival tissue color with pink porcelain. Therefore, on many occasions, the available dental porcelain systems constitute the reference used as a gingival color guide.
      The main null hypothesis was accepted because none of the 3 variables (age, sex, and professional category) significantly influenced the degree of concordance between the arrangements proposed by the participants and the ideal arrangement of the pink ceramic specimens, in terms of lightness. However, the secondary null hypothesis was rejected because the darkest pink porcelain specimens were arranged significantly better according to their lightness than the lightest specimens.
      The decision to study lightness was based on a protocol through which dental color is often selected in clinical practice, where lightness is determined first, followed by chroma and hue.
      • Sakaguchi R.L.
      • Ferracane J.
      • Powers J.
      Craig's restorative dental materials.
      The Kendall correlation coefficient T evaluated the degree of agreement of the specimen orderings, sorted by the participants according to their lightness (which was influenced not only by the L∗ coordinate but also by the coordinates a∗ and b∗), with the ordering obtained based solely on the L∗ coordinate (the ideal arrangement). Therefore, the coefficient T assessed the influence that the coordinates a∗ and b∗ had on the perception of the lightness of the specimens. Future studies should quantify the influence of the coordinates a∗ and b∗ on the selection of gingival color. The thresholds of gingival chromatic perception and acceptance should also be studied to be able to design gingival guides with a comprehensive mathematical criterion. Although there are no spectrophotometers marketed to measure gingival color, the SpectroShade Micro was used as it has been used in tooth color studies.
      • Gómez-Polo C.
      • Montero J.
      • Gómez-Polo M.
      • Martín Casado A.M.
      Clinical study on natural gingival color.
      ,
      • Bressan E.
      • Paniz G.
      • Lops D.
      • Corazza B.
      • Romeo E.
      • Favero G.
      Influence of abutment material on the gingival color of implant-supported all-ceramic restorations: a prospective multicenter study.
      ,
      • Happe A.
      • Schulte-Mattler V.
      • Fickl S.
      • Naumann M.
      • Zöller J.E.
      • Rothamel D.
      Spectrophotometric assessment of peri-implant mucosa after restoration with zirconia abutments veneered with fluorescent ceramic: a controlled, retrospective clinical study.
      ,
      • Jung R.E.
      • Sailer I.
      • Hämmerle C.H.
      • Attin T.
      • Schmidlin P.
      In vitro color changes of soft tissues caused by restorative materials.
      • Kim A.
      • Campbell S.D.
      • Viana M.A.
      • Knoernschild K.L.
      Abutment material effect on peri-implant soft tissue color and perceived esthetics.
      • Valente N.A.
      • Sailer I.
      • Fehmer V.
      • Thoma D.S.
      Color differences between pink veneering ceramics and the human gingiva.
      • Sailer I.
      • Fehmer V.
      • Ioannidis A.
      • Hämmerle C.H.
      • Thoma D.S.
      Threshold value for the perception of color changes of human gingiva.
      • Zembic A.
      • Sailer I.
      • Jung R.E.
      • Hämmerle C.H.
      Randomized-controlled clinical trial of customized zirconia and titanium implant abutments for single-tooth implants in canine and posterior regions: 3-year results.
      The results of this study suggest that all the participants were able to order pink specimens correctly and consistently according to lightness (Tables 2 and 3). In addition, it was found that specific training in dental color matching did not necessarily improve the ability to distinguish among the varying degrees of lightness of gingival ceramics. In fact, experienced dentists tended to be less consistent when identifying a range of light colors (Table 4). The T values in Table 5 were slightly smaller than those in Table 3 but greater than those in Table 4, which indicates that the raters were more accurate in ranking the darker shade tabs than the lighter ones. This may occur because the differences in lightness among the 10 darker tabs were larger than the differences in lightness among the 10 lighter tabs.
      Other authors have also pointed out that dental work experience does not influence color matching abilities.
      • Sinmazisik G.
      • Trakyali G.
      • Tarcin B.
      Evaluating the ability of dental technician students and graduate dentists to match tooth color.
      ,
      • Curd F.M.
      • Jasinevicius T.R.
      • Graves A.
      • Cox V.
      • Sadan A.
      Comparison of the shade matching ability of dental students using two light sources.
      ,
      • Thoma D.S.
      • Ioannidis A.
      • Fehmer V.
      • Michelotti G.
      • Jung R.E.
      • Sailer I.
      Threshold values for the perception of color changes in human teeth.
      This relatively unexpected result may be explained by the fact that continued exposure of dentists to LEDs may cause them to be insensitive to the detection of subtle differences in lightness, due to potential damage to the retina or fatigue of the retinal photoreceptor cells.
      • Bruzell Roll E.M.
      • Jacobsen N.
      • Hensten-Pettersen A.
      Health hazards associated with curing light in the dental clinic.
      • Stamatacos C.
      • Harrison J.L.
      The possible ocular hazards of LED dental illumination applications.
      • Price R.B.
      • Labrie D.
      • Bruzell E.M.
      • Sliney D.H.
      • Strassler H.E.
      The dental curing light: a potential health risk.
      • Labrie D.
      • Moe J.
      • Price R.B.
      • Young M.E.
      • Felix C.M.
      Evaluation of ocular hazards from 4 types of curing lights.
      However, other studies reported that dentists are better at selecting color than other professional categories considered
      • Capa N.
      • Malkondu O.
      • Kazazoglu E.
      • Calikkocaoglu S.
      Evaluating factors that affect the shade-matching ability of dentists, dental staff members and laypeople.
      and that work experience also had an influence.
      • Capa N.
      • Malkondu O.
      • Kazazoglu E.
      • Calikkocaoglu S.
      Evaluating factors that affect the shade-matching ability of dentists, dental staff members and laypeople.
      Women were more accurate at sorting the shade tabs than men (Table 3), consistent with other studies.
      • Haddad H.J.
      • Jakstat H.A.
      • Arnetzl G.
      • Borbely J.
      • Vichi A.
      • Dumfahrt H.
      • et al.
      Does gender and experience influence shade matching quality?.
      ,
      • Gómez-Polo C.
      • Montero J.
      • Gómez-Polo M.
      • Martin Casado A.M.
      Comparison of the CIELab and CIEDE 2000 color difference formulas on gingival color space.
      Additional studies of this type are needed, as wider knowledge regarding the perception of the gingival chromatic space will help to optimize the subjective selection of gingival color.

      Conclusions

      Based on the findings of this in vitro study, the following conclusions were drawn:
      • 1.
        There were no statistically significant differences among dentists, dental assistants, dental students, and laypersons with respect to the ranking of colors in the gingival chromatic space based on lightness.
      • 2.
        The ranking of the darker gingival specimens was significantly more accurate than that of the lighter gingival specimens, irrespective of sex, dental work experience, or professional category.

      Acknowledgments

      The authors thank the research group named Avances en Salud Oral of Department of Surgery at the University of Salamanca for the institutional support provided for this research project and Elena P. Hernández Rivero (Central Language Service-USAL) for translation and linguistic review. The authors would also like to thank the work of the dental laboratory Dentislab for the design of the gingival porcelain specimens.

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