Abstract
Statement of problem
Dental implants obtained by additive manufacturing may present changes in the microbiome
formed. However, studies profiling the microbial communities formed on Ti-6Al-4V are
lacking.
Purpose
The purpose of this in situ study was to characterize the profile of the microbial
communities formed on Ti-6Al-4V disks produced by additive manufacturing and machining.
Material and methods
Titanium disks produced by additive manufacturing (AMD) and machining (UD) were housed
in the buccal region of removable intraoral devices. These devices containing both
disks were used by eight participants for 96 hours. After every 24 hours of intraoral
exposure, the biofilm that had formed on the disks was collected. The 16S rRNA genes
from each specimen were amplified and sequenced with the Miseq Illumina instrument
and analyzed. Total microbial quantification was evaluated by analysis of variance-type
statistics using the nparLD package. The Wilcoxon test was used to evaluate alpha
diversity (α=.05).
Results
A difference was found in the microbial communities formed on additively manufactured
and machined disks, with a reduction in operational taxonomic units (OTUs) for the
AMD group compared with the UD group. Firmicutes and Proteobacteria were the most abundant phyla. Of the 1256 genera sequenced, Streptococcus predominated on both disks.
Conclusions
The microbiome of the biofilm formed on the Ti-6Al-4V disks was significantly influenced
by the fabrication method. The AMD disks showed lower total microbial counts than
the UD disks.
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Article info
Publication history
Published online: April 28, 2023
Publication stage
In Press Corrected ProofFootnotes
Funding: Supported by a grant from Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP [Processes 2017/17012–2 and 2017/10336–7].
Identification
Copyright
© 2023 by the Editorial Council for The Journal of Prosthetic Dentistry.
